N3-kethoxal (also known as azide kethoxal N3-kethoxal) is a small molecule probe of guanine residues in single-stranded DNA and RNA, which can be used for bioorthogonal click chemistry reactions in genome-wide methods such as KAS-seq and Keth-seq.
N3-kethoxal reacts with guanosines in single-stranded DNA (ssDNA) and single-stranded RNA (ssRNA) and has a dicarbonyl structure that adopts a hydrate form in biological assays. AccuraDX holds the exclusive license to the molecule, which was invented at The University of Chicago. AccuraDX draws on KAS-seq and keth-seq expertise for N3-kethoxal synthesis and chemical characterization for its optimal stability and reproducibility.
AccuraDX N3-kethoxal is formulated for your ssDNA and ssRNA labeling needs in dimethyl sulfoxide (DMSO). To follow the KAS-seq and keth-seq protocols, AccuraDX recommends the 500 mM solution available here. If you have custom N3-kethoxal concentration needs, please contact AccuraDX at firstname.lastname@example.org.
Recommended storage conditions: Aliquot into small volumes based on your experimental needs and store at -20 C.